HomeCzechia-Startups10 innovative Czech startups leading the charge in 2025!

10 innovative Czech startups leading the charge in 2025!

As 2025 unfolds, a new wave of Czech startups is ready to make its mark, bringing fresh ideas, innovative technologies, and an unstoppable entrepreneurial drive. These emerging companies are not just entering the scene; they’re set to disrupt industries, further establishing the Czech Republic as a leading destination for startups in the region.

In recent years, the country has been drawing the attention of both investors and entrepreneurs. Cities like Prague, Brno, and Ostrava want to become key centres of innovation in Central and Eastern Europe, particularly in fields like ICT, software development, and mobile apps. To nurture this growth, the Czech Startup Association was created, aiming to ensure a supportive environment that helps startups thrive and keeps the country at the cutting edge of the global market.

In this article, we delve into the Czech entrepreneurial scene to highlight 10 promising startups, all founded after 2022, that are likely to make a notable impact across different sectors.

Adalid Sciences

Adalid Sciences: Founded in 2024 and based in Prague, this biotechnology startup is dedicated to advancing pharmaceutical solutions through cutting-edge lipid nanoparticle (LNP) technology. By leveraging LNPs, the company aims to enhance drug stability and bioavailability, improving therapeutic efficacy while minimising side effects.

With extensive expertise in synthetic chemistry, biochemistry, and nanoparticle formulation, Adalid Sciences seamlessly integrates its solutions into client workflows. They have secured over €8 million in funding to drive innovation in nucleic acid delivery systems and related fields.

cruxo

CRUXO: This data-driven startup has developed an innovative platform designed to help retailers boost their revenue by automating advertising and providing real-time insights. It simplifies managing targeted ads, boosts shopper engagement, and increases earnings from on-site media in sectors like beauty, electronics, and groceries. Founded in 2022 in Prague, the startup has raised €800k to leverage expertise in retail, vendor marketing, and online media to deliver innovative solutions for businesses.

flowlance

Flowlance: Launched in 2022, Flowlance is a platform designed to help creators turn their knowledge into income effortlessly. It allows users to sell services, digital products, and memberships, manage bookings, collect payments, and build a professional online presence, all from one platform. With easy-to-use tools, creators can launch a website, track sales, and optimise their business growth without needing technical skills. Based in Prague they have raised 500k in funding.

kardi

Kardi AI: Based in Olomouc, Kardi AI focuses on providing worry-free heart care from home. Kardi AI combines commercially available trackers with cutting-edge AI to monitor heart health, making it easier for individuals to track and manage their cardiovascular well-being.

Through AI, machine learning, and healthcare, the pioneering startup aims to transform the way heart health is monitored outside of medical facilities. Founded in 2022 and with a funding of €1.9 million, Kardi AI is positioning itself as a leader in remote heart care, ensuring early detection and continuous monitoring for users.

lakmoos

Lakmoos: This market research startup uses neuro-symbolic AI to simulate consumer behaviour. Lakmoos platform provides instant, accurate market insights, enabling businesses to gather real-time data without the need for human respondents or long, costly research processes. By leveraging AI, they offer a cost-effective, fast, and secure solution for industries like automotive, banking, and telecommunications.

Headquartered in Brno, Lakmoos aims to disrupt traditional market research with an automated, data-driven approach to consumer insights. Launched in 2023, the startup landed €510k in funding to fuel its mission of transforming how businesses gather consumer data.

langtail

Langtail: Prague-based, Langtail is a platform that simplifies the process of testing AI applications, especially those that use large language models. Founded in 2023, their innovative platform helps teams ensure their AI works well by testing its responses and improving accuracy, all through a simple interface. This makes enhancing AI security and performance easier for consumers, even those without extensive technical understanding. Langtail has successfully raised €954k.

merchantee

Merchantee: Launched in 2023, Merchantee is an e-commerce SaaS platform designed to help online sellers manage their sales across EU marketplaces. Based in Prague, Merchantee simplifies the complexities of multi-marketplace selling, allowing businesses to seamlessly track, manage, and improve their sales in one unified platform. With a funding of €614k, the innovative startup is empowering e-commerce entrepreneurs to expand their reach and increase operational efficiency across the European Union’s diverse online markets.

Rezolv Energy

Rezolv Energy: Based in Prague, this independent renewable energy producer focuses on wind, solar, and battery storage solutions. The startup provides subsidy-free renewable energy at stable prices for industrial and commercial users, ensuring access to reliable sustainable power. Founded in 2022, Rezolv Energy has raised €90 million, marking significant investor confidence in its mission to build a sustainable power portfolio across Central and South Eastern Europe.

robosize

RobosizeME: Founded in 2022, this automation-focused startup is revolutionising operational processes in the hospitality industry by streamlining repetitive tasks. With its automation as a service (AaaS) model, the startup handles everything from identifying automation opportunities to building, implementing, and maintaining solutions. By automating tasks such as rate loading, commission tracking, and payment reconciliation, the startup enables hotel staff to focus on delivering better guest experiences. The Olomouc-based startup has raised €1 million to scale its innovative automation services.

salted

Salted CX: Based in Prague, Salted CX assists companies in providing better customer service. By using AI, they centralise data from all customer interactions either, chats, emails or phone calls, to analyse each discussion, identify trends, and point out areas for improvement.

The platform makes it simple to track performance, look for specific issues, and generate reports to identify issues quickly. For instance, it can highlight areas where clients are irritated or where agents require more assistance. In this manner, companies may maintain customer satisfaction, enhance service, and resolve problems more quickly. Founded in 2022, Salted CX has raised €1.4 million to develop its software solutions.

By the way: If you’re a corporate or investor looking for exciting startups in a specific market for a potential investment or acquisition, check out our Startup Sourcing Service!3

Olga G. Codina
Olga G. Codina
Olga is the Startup Analyst at EU-Startups, specialising in disruptive innovation and emerging technologies. With a background in International Relations, she focuses on identifying startups with the potential to transform industries. Her analytical approach, along with a strong interest in how technology's influence the future, generates her commitment to supporting innovative ideas.
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